Hayes’ side go into the game two points behind the Italian champions in Group A after drawing their opening match at home to Wolfsburg last week.
The clash will see her come up against former Arsenal Women manager Joe Montemurro for the first time since he ended his four-year spell in north London to take charge at Juventus in the summer.
And Hayes is looking forward to taking on her former rival again, saying: “This is Italy, it’s an amazing place to play football and an opponent we haven’t played in the Champions League yet.
“So I think [there is] intrigue and interest, but a coach that I know very well and I’m looking forward to seeing Joe tomorrow in what is always an epic battle between us both.”
Hayes – who guided Chelsea to the Women’s Champions League final last season – has also warned her side to expect a “tense atmosphere” in Turin for what could be a key game in deciding qualification for the knockout stages.
“My number one goal is winning tomorrow night,” Hayes said. “It’s an amazing opportunity to come to Turin to play Juventus. We expect a crowd, expect a tense atmosphere.”
But Hayes – whose side sit second in the Women’s Super League – insists she and her players relish European nights, adding: “It’s just another great game, we’ve already had good tests so far.
“I know we’ve got a dressing room that are really enjoying playing in the Champions League and we’re just looking forward to pitting ourselves against the top team in Italy.
“I certainly get the most joy from playing against teams I don’t know too much about.
“Obviously I know Joe and I’m looking forward to seeing him tomorrow and saying ‘Hi’ and I’m sure he’ll bring a lot of success to this football club.”
Chelsea defender Magdalena Eriksson backed up her manager’s comments, saying: “Last time we played Juventus was three or four years ago, so you don’t play them very often which makes it even more special.
“It’s exciting to see where you’re at compared to other top teams in other top leagues, so I’m excited by that.”